Denaturation protein

Conformation (qaab-dhismeedka) of silsilad peptide la amartay oo gaar u tahay protein kasta. In xaaladaha gaarka ah uu jiro farqi tiro badan oo ah bonds oo xasilin dhismeedka aan ollogga ah ee Britain xarunta. Sidaas darteed, farqiga u gebi ahaanba Britain oo dhan (ama qaybta la taaban karo oo dhanba) qaadataa foomka of gariiradda random. Nidaamkan waxaa loo yaqaan "denaturation". Isbeddelkan ayaa kicin kara kulaylka ka lixdan in siddeetan degrees. Sayidka, Britain kasta, farqiga u keentay kala duwanaan karaan in conformation kale.

Denaturation borotiinada qaadataa meel sarkhaansan yihiin wakiilada kasta oo awood u baabbi'iyeen bonds non-covalent. Hawlgalkani waxa dhici kara xaalado xumadoodu ama acidic, oo korka ka mid ah qaybta wajiga, hoos tallaabo ah ee qaar ka mid ah xeryahooda organic (phenols, alcohols iyo kuwo kale). denaturation protein dhici karaan sarkhaansan chloride guanidinium ama HOL. wakiilada ayaa sheegay in ay u sameeyaan bonds daciif ah (hydrophobic, Madbacada qaranka, hydrogen) la carbonyl ama amino kooxaha laf peptide la tiro ka mid ah kooxaha ku harta amino acid ah, in lagu badalaa heli protein iyaga u gaar ah bonds hydrogen taagoo gudahood. Sidaas darteed, isbedel ku dhacdaa qaab-dhismeedka Dugsiyada Sare iyo Jaamacadaha ee.

Resistance wakiilada denaturing ku xiran tahay inta ay joogaan in Britain ee xeryahooda protein silsilado disulfide ah. inhibitor Trypsin waxay leedahay saddex S bonds - S. Under xaaladda kabashada denaturation protein dhacdaa iyada oo aan saamayn kale. Haddii danbe saaray xidhiidhka xaaladaha halkaas oo lagu qayilo waxaa fuliyay SH-kooxood oo cysteine iyo formation of bonds disulfide, conformation asalka waa lay soo celiyey. Joogitaanka xitaa hal bond disulfide a si weyn u kordhiyaa xasiloonida ee qaab-dhismeedka da'ayeen.

denaturation protein, caadi ahaan ay la socdaan hoos u dhac in milmi. Wada jir ah ula waxa ay inta badan soo dadajin foomamka. Waxay dhacdaa qaab "protein gadhoodh." At sare ee xeryahooda ee xalka, "xinjirowga" Oo Qaliin xalka oo dhan, sida, tusaale ahaan, tani dhacdaa marka ukun karkaraya. Marka denaturation of protein ayaa lumiya hawl noolaha. Mabda'ani waxa uu ku salaysan isticmaalka acid carbolic (phenol xal aqueous) sida walax aalkolada.

xasillooni darrada ah ee qaab-dhismeedka da'ayeen, jaaniska sare ee failure sarkhaansan yihiin wakiilada kala duwan uga adag tahay in la go'doomiyo iyo cilmi-protein. Sidoo kale abuuray dhibaatooyin qaar ka mid ah marka la isticmaalayo xeryahooda ee daawada iyo warshadaha.

Haddii denaturation protein la fuliyaa by gaadhista heerkulka sare, qaboojinta gaabis meel shuruudo gaar ah renativatsii habka - kabashada (asalka) conformation hooyo ah. Dhab ahaantii waxa uu xaqiijinayaa in la dul saarin of silsilad peptide waa iyada oo la raacayo qaab dhismeedka hoose.

Aasaasidda conformation hooyo (meel il) waa habka shaqo. In si kale loo dhigo, nidaamka this u dhiganta lacagta ugu yar ee tamarta free ku jira Britain ah. Mid ka mid ah iyada oo sababtu tahay in lagu soo gabagabeeyey karaa in qaab-dhismeedka aan ollogga ah xarunta la encoded ee isku xigxiga amino acid ee silsilado peptide ah. Tani waxa ay noqonaysaa ka dhigan tahay in dhammaan polypeptides la xiriira by iskhilaafka of acids amino (tusaale ahaan, silsilado peptide mioglobinovye), qaadan doonaa conformation ah isku.

Borotiin noqon kartaa farqi weyn oo qaab-dhismeedka aasaasiga ah, xitaa haddii ay wax yar ama gabi ahaanba isku mid in conformation.